Chaetomium globosum CDW7，老人护理13825404095一种潜在的生物防治菌株及其抗真菌代谢产物。
FEMS Microbiology Letters
(
IF
2.2
)
Pub Date : 2016-12-25
, DOI:
10.1093/femsle/fnw287
Shuang-Shuang Zhao
1,
2
,
Ying-Ying Zhang
1,
2
,
Wei Yan
1,
2
,
Ling-Ling Cao
1,
2
,
Yu Xiao
1,
2
,
Yong-Hao Ye
1,
2
Affiliation
College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, P.R. China.
Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P.R. China.
筛选具有抗真菌活性的内生真菌是发现新型生物农药的有效策略。我们以前的工作表明，银杏内生菌Chaetomium globosum CDW7在体外对植物病原真菌具有很强的抑制活性。在这项研究中，我们评估了CDW7菌株对9种植物病原真菌的抗真菌活性以及对由菌核盘菌引起的油菜菌核盘菌腐烂的生物防治潜力。CDW7的发酵液可以成功抑制体内被链球菌感染的油菜的疾病发展，具有57.8％的保护效率，与250μgmL-1的多菌灵（59.8％）相当。当暴露于60°C和UV照射下或储存于4°C时，发酵液还表现出明显的活性稳定性。此外，我们发现10％的发酵液可以促进油菜幼苗的发芽和生长。紧接着以生物测定为指导的方法，分离了7种已知的代谢物，并通过光谱分析进行了鉴定。其中，chaetoglobosin A和D对菌核菌具有抑制活性，与多菌灵（0.17μgmL-1）相比，IC50值分别为0.35和0.62μgmL-1。因此，我们的研究表明，CDW7是一种有希望的农业防治菌核菌的生物防治真菌。与多菌灵（0.17μgmL-1）相比，菌核菌的IC50值分别为0.35和0.62μgmL-1。因此，我们的研究表明，CDW7是一种有希望的农业防治菌核菌的生物防治真菌。与多菌灵（0.17μgmL-1）相比，菌核菌的IC50值分别为0.35和0.62μgmL-1。因此，我们的研究表明，CDW7是一种有希望的农业防治菌核菌的生物防治真菌。
Chaetomium globosum CDW7, a potential biological control strain and its antifungal metabolites.
Screening for endophytic fungi with antifungal activity is an effective strategy for the discovery of novel biopesticides. Our previous work indicated that Chaetomium globosum CDW7, an endophyte from Ginkgo biloba, exhibited strong inhibitory activity against plant pathogenic fungi in vitro. In this study, we evaluated the CDW7 strain for its antifungal activities against nine phytopathogenic fungi and its biocontrol potential against rape sclerotinia rot caused by Sclerotinia sclerotiorum. The fermentation broth of CDW7 could successfully inhibit disease development in S. sclerotiorum-infected rape in vivo with 57.8% protective efficiency, which is comparable to that of carbendazim (59.8%) at 250 μg mL-1. The fermentation broth also expressed significant activity stability when exposed to 60°C and UV illumination, or when stored at 4°C. Furthermore, we found that 10% fermentation broth can promote the germination and growth of rape seedlings. Followed by the bioassay-guided approach, seven known metabolites were isolated and identified by spectroscopic analyses. Among them, chaetoglobosin A and D exhibited inhibitory activity against S. sclerotiorum with IC50 values of 0.35 and 0.62 μg mL-1, respectively, compared with carbendazim (0.17 μg mL-1). Therefore, our study demonstrated that CDW7 is a promising biocontrol fungus against S. sclerotiorum in agriculture.
