四种生防菌的发酵优化、抑菌作用及促番茄生长
Agriculture
(
IF
2.072
)
Pub Date : 2021-07-20
, DOI:
10.3390/agriculture11070686
Yao Zhang
,
Xingyuan Wang
,
Sibo Liang
,
Yuying Shi
,
Xiuling Chen
,
Jiayin Liu
,
Aoxue Wang
番茄是一种广泛种植的作物，接送孩子13825404095因其营养价值和遗传多样性非常重要。番茄生产受到病虫害的严重挑战，其中番茄灰霉病和叶霉病尤为严重。生物防治是番茄生产中最优选的疾病管理方法之一。目前用于防治番茄灰霉病的真菌主要是木霉和酵母菌。芽孢杆菌和放线菌是防治番茄叶霉病最有效的微生物。番茄灰霉病和叶霉病在生产过程中经常同时发生，但同时防治这两种病害的菌株较少。生物防治细菌偶氮假单胞菌 WXCDD51，芽孢杆菌属。WXCDD105，枯草芽孢杆菌BS和解淀粉芽孢杆菌上一阶段分离筛选出的BS WY-1，对番茄灰霉病和叶霉病都有预防作用。在这里，我们一起优化了四种生防菌株的液体发酵。我们获得了四种生防菌的最佳发酵培养基配方和发酵条件。测试了四种生防菌的广谱特性，并在此基础上构建了复合菌株。评价了单株和复合株对番茄灰霉病和叶霉病的防治效果。还研究了它们对番茄种子和幼苗生长的潜在影响。该研究为开发和使用复合细菌在番茄生产中促进生长和疾病管理提供了基础。
Fermentation Optimization, Fungistatic Effects and Tomato Growth Promotion of Four Biocontrol Bacterial Strains
Tomato is a widely cultivated crop that is important for its nutritional value and genetic diversity. Tomato production is seriously challenged by pests and diseases, among which tomato gray mold and leaf mold are particularly serious. Biological control is one of the most preferred methods for disease management in tomato production. At present, the fungi used to control tomato gray mold are mainly Trichoderma and yeast. Bacillus and actinomycetes are the most effective microorganisms for controlling tomato leaf mold. Tomato gray mold and leaf mold often occur at the same time during the production process, yet there are fewer strains for controlling both diseases at the same time. Biocontrol bacteria Pseudomonas azotoformans WXCDD51, Bacillus sp. WXCDD105, Bacillus subtilis BS and Bacillus amyloliquefaciens BS WY-1, which were isolated and screened in the previous stage, can prevent both tomato gray mold and leaf mold. Here, we optimized liquid fermentation for the four biocontrol bacterial strains together. We obtained the best fermentation medium formula and fermentation conditions for the four biocontrol bacteria. The broad-spectrum properties of the four biocontrol bacteria were tested, and, on this basis, compound strains were constructed. The control effect of single and compound strains on tomato gray mold and leaf mold was evaluated. Their potential effects on the growth of tomato seeds and seedlings were also studied. This research provides a foundation for the development and use of compound bacteria for growth promotion and disease management in tomato production.
